Djurup R, Chiabrando C, Jörres A, Fanelli R, Foegh M, Soerensen H U, Joergensen P N
Novo Nordisk, Immunochemical Department, Bagsvaerd, Denmark.
Clin Chem. 1993 Dec;39(12):2470-7.
We have developed a direct enzyme immunoassay (EIA) for quantifying immunoreactive 11-keto-thromboxane B2 (iKTXB) in unprocessed human urine. Cross-reactivity with other thromboxane metabolites and prostanoids was negligible. Analytical recovery of 11-keto-TXB2 in urine specimens was 97.4% to 99.8%. Total imprecision for two clinical specimens was 8.5% and 12.2%. Intake of acetylsalicylic acid decreased the measured concentration of iKTXB. Cardiopulmonary bypass, a procedure known to activate platelets, increased the mean excretion rate of iKTXB 10-fold. Simultaneous gas chromatography-mass spectrometry analysis of 11-keto-TXB2 and 11-keto-2,3-dinor TXB2 in urine specimens (n = 17) from healthy subjects indicated that urinary iKTXB concentrations measured by EIA represented a sum of the two 11-keto metabolites. We conclude that the direct EIA is sufficiently sensitive, rapid, simple, and specific to allow screening for alterations in thromboxane biosynthesis in patients.
我们开发了一种直接酶免疫测定法（EIA），用于定量未经处理的人尿液中免疫反应性11-酮血栓素B2（iKTXB）。与其他血栓素代谢物和前列腺素的交叉反应可忽略不计。尿液标本中11-酮-TXB2的分析回收率为97.4%至99.8%。两个临床标本的总不精密度分别为8.5%和12.2%。摄入乙酰水杨酸会降低iKTXB的测量浓度。体外循环是一种已知会激活血小板的手术，它使iKTXB的平均排泄率增加了10倍。对健康受试者尿液标本（n = 17）中的11-酮-TXB2和11-酮-2,3-二去甲TXB2进行气相色谱-质谱联用分析表明，通过EIA测量的尿液iKTXB浓度代表了这两种11-酮代谢物的总和。我们得出结论，直接EIA足够灵敏、快速、简单且特异，可用于筛查患者血栓素生物合成的改变。
