Rosenberg C, Dénarié J
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Mu cts 62 a thermo-inductible mutant of phage Mu was integrated in E. coli in to the broad host range RP4 plasmid. The hybrid plasmid RP4::Mu cts 62 was then transferred by mating to the dinitrogen-fixing strain K. pneumoniae M5al. In Klebsiella Mu cts 62 is still heat inducible and the phage production is similar to that observed in E. coli. Mu Should be a useful tool for the genetic analysis of nitrogen fixation.
噬菌体 Mu 的热诱导突变体 Mu cts 62 整合到大肠杆菌的广宿主范围 RP4 质粒中。然后通过接合将杂种质粒 RP4::Mu cts 62 转移到固氮菌株肺炎克雷伯氏菌 M5al 中。在克雷伯氏菌中,Mu cts 62 仍然是热诱导的,并且噬菌体产量与在大肠杆菌中观察到的相似。Mu 应该是用于固氮遗传分析的有用工具。