利用噬菌体Mu分离肺炎克雷伯菌his-nif区域的缺失突变体。
Use of bacteriophage Mu to isolate deletions in the his-nif region of Klebsiella pneumoniae.
作者信息
Bachhuber M, Brill W J, Howe M M
出版信息
J Bacteriol. 1976 Dec;128(3):749-53. doi: 10.1128/jb.128.3.749-753.1976.
Abstract
Klebsiella pneumoniae M5a1 is naturally resistant to infection by bacteriophage Mu. Mutants of K. pneumoniae sensitive to Mu infection were isolated and found to support both lytic and lysogenic development of Mu. K. pneumoniae lysogens containing a heat-inducible Mu prophage integrated in his were isolated. Strains carrying deletions extending from his into nif were obtained after heat treatment of these lysogens. Such deletions should be useful for determining the map order and cistronic organization of the nif genes.
摘要
肺炎克雷伯菌M5a1对噬菌体Mu的感染具有天然抗性。分离出了对Mu感染敏感的肺炎克雷伯菌突变体，发现它们支持Mu的裂解性和溶原性发育。分离出了含有整合在his中的热诱导型Mu原噬菌体的肺炎克雷伯菌溶原菌。对这些溶原菌进行热处理后，获得了从his延伸到nif的缺失菌株。这种缺失对于确定nif基因的图谱顺序和顺反子组织应该是有用的。
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