将固氮细菌诱变使其对噬菌体Mu感染敏感：由Mu诱导的肺炎克雷伯菌M5al的固氮基因（nif）突变的分离
Mutational alteration of a nitrogen-fixing bacterium to sensitivity to infection by bacteriophage Mu: isolation of nif mutations of Klebsiella pneumoniae M5al induced by Mu.
作者信息
Rao R N
出版信息
J Bacteriol. 1976 Oct;128(1):356-62. doi: 10.1128/jb.128.1.356-362.1976.
Abstract
The nitrogen-fixing bacterium Klebsiella pneumoniae M5al is not sensitive to infection by bacteriophage Mu. A mutant of K. pneumoniae that is sensitive to Mu infection was isolated. Several Mu-induced auxotrophic mutations of K. pneumoniae including nif, trp, and rtl were isolated and genetically characterized. Evidence is presented that the Mu-induced mutations of nif arise as the result of insertion of Mu within (or near) the nif operon(s). The rtl locus, which determines the ability to utilize ribitol as a carbon source, was found to be linked to nif loci.
摘要
固氮细菌肺炎克雷伯氏菌M5al对噬菌体Mu的感染不敏感。分离出了一株对Mu感染敏感的肺炎克雷伯氏菌突变体。还分离出了肺炎克雷伯氏菌的几个由Mu诱导的营养缺陷型突变，包括nif、trp和rtl，并对其进行了遗传学特征分析。有证据表明，nif的Mu诱导突变是Mu插入nif操纵子（或其附近）的结果。发现决定利用核糖醇作为碳源能力的rtl位点与nif位点相连。
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