Kyes S, Craig A G, Marsh K, Newbold C I
Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, England, U.K.
Exp Parasitol. 1993 Dec;77(4):473-83. doi: 10.1006/expr.1993.1108.
Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates.
聚合酶链反应扩增多个多态性基因已被用于研究恶性疟原虫的群体生物学。S抗原特别适合此类研究，但该基因扩增的困难阻碍了其应用。在此，我们描述了一种扩增S抗原的简单方法，并说明先前尝试可能未成功的原因。文中展示了来自实验室分离株和现场分离株的数据。
