Kuchkina N V, Orlov S N, Pokudin N I, Chuchalin A G
Department of Biology, Moscow State University, Russia.
Experientia. 1993 Nov 15;49(11):995-7. doi: 10.1007/BF02125647.
The effect of incubation medium osmolality on the respiratory burst of human neutrophils was studied using luminol-dependent chemiluminescence (CL) as an indicator of burst activity. Neutrophils were stimulated with N-formyl-Met-Leu-Phe (FMLP), phorbol-12-myristate-13-acetate (PMA), the calcium ionophore A23187, thermoaggregated IgG (IgGn), and opsonized zymosan (OZ). It was shown that increasing the osmolality of the incubation medium from 320 up to 420 mosM decreased the A23187- and OZ-induced CL responses by 90%. Under the same conditions PMA-, FMLP- and IgGn-induced CL responses were decreased by 40-60%. A decrease of osmolality to 200 mosM resulted in a 2-3 fold decrease of the A23187-, PMA- and FMLP-induced CL and in a 60-80% increase of OZ- and IgGn-induced CL. It is suggested that osmolality-mediated alteration of cell volume is an important mechanism for regulating neutrophil activity.
使用鲁米诺依赖性化学发光（CL）作为爆发活性的指标，研究了孵育培养基渗透压对人中性粒细胞呼吸爆发的影响。用N-甲酰甲硫氨酸亮氨酸苯丙氨酸（FMLP）、佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）、钙离子载体A23187、热聚集IgG（IgGn）和调理酵母聚糖（OZ）刺激中性粒细胞。结果表明，将孵育培养基的渗透压从320 mosM提高到420 mosM，可使A23187和OZ诱导的CL反应降低90%。在相同条件下，PMA、FMLP和IgGn诱导的CL反应降低40-60%。将渗透压降至200 mosM会导致A23187、PMA和FMLP诱导的CL降低2-3倍，以及OZ和IgGn诱导的CL增加60-80%。提示渗透压介导的细胞体积改变是调节中性粒细胞活性的重要机制。
