小鼠B淋巴细胞的个体发育。通过免疫过氧化物酶测定法对表面膜免疫球蛋白进行定量分析。
Ontogeny of B lymphocytes in mice. Quantification of surface membrane immunoglobulins by immunoperoxidase assay.
作者信息
Follezou J Y, Dighiero G, Roisin J P, Binet J L
出版信息
Immunology. 1978 Apr;34(4):733-9.
Surface membrane immunoglobulins (SmIg) of splenic lymphocytes were investigated by immunoperoxidase assay in newborn Swiss mice, their mothers and adult controls. The mean number of SmIg + cells in adult mice was 45-8% and the mean number of antigenic sites per positive cells was 108,500. In newborn mice during the first 7 days of life, values for both parameters were low (24-25--28-5% and 38,000-45,773 respectively) and began to rise after the 10th day to reach adult levels between the ages of 2 and 3 weeks. A peak above adult levels for the mean number of sites per cell was observed on day 21 with a subsequent drop to adult levels on day 28. Post-partum females were found to have consistently fewer sites per positive cell (69,000-86,600) during the 14 days following delivery than their adult control counterparts, though the difference was not statistically significant.
采用免疫过氧化物酶分析法，对新生瑞士小鼠、其母鼠及成年对照鼠脾脏淋巴细胞的表面膜免疫球蛋白（SmIg）进行了研究。成年小鼠中SmIg+细胞的平均数量为45 - 8%，每个阳性细胞的抗原位点平均数量为108,500个。在出生后第1周内的新生小鼠中，这两个参数的值均较低（分别为24 - 25 - 28 - 5%和38,000 - 45,773），在第10天后开始上升，在2至3周龄时达到成年水平。在第21天观察到每个细胞的位点平均数量出现高于成年水平的峰值，随后在第28天降至成年水平。产后母鼠在分娩后的14天内，每个阳性细胞的位点数量始终比成年对照鼠少（69,000 - 86,600），尽管差异无统计学意义。
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