Dighiero G, Bodega E, Mayzner R, Binet J L
Blood Cells. 1980;6(3):371-9.
Two new applications of the immunoperoxidase method are reported. In the first one, cells after incubation with antibodies coupled with peroxidase washing and staining by carbazol are cytocentrifuged and individually analyzed for their content on surface immunoglobulin (Ig) in two photometric microscopes. The results included the following: 1. Maturation of mice B-lymphocytes during ontogeny is accompanied by the appearance of a population containing intermediate to high amounts of SIg. 2. The CLL B-lymphocyte pattern as compared with that of normal B-lymphocytes shows a decrease of SIg and a homogeneous distribution. In the second application, mice splenic lymphocytes are incubated with antimouse Ig coupled with peroxidase and analyzed through Hemalog D. B-lymphocytes are separated from T-lymphocytes in a distinct population since they become positive for peroxidase.
报道了免疫过氧化物酶方法的两种新应用。在第一种应用中，细胞与偶联过氧化物酶的抗体孵育后，经过洗涤并用咔唑染色，然后进行细胞离心涂片，并在两台光度显微镜下分别分析其表面免疫球蛋白（Ig）含量。结果如下：1. 小鼠B淋巴细胞在个体发育过程中的成熟伴随着出现一群含有中等到大量表面免疫球蛋白的细胞。2. 与正常B淋巴细胞相比，慢性淋巴细胞白血病B淋巴细胞模式显示表面免疫球蛋白减少且分布均匀。在第二种应用中，将小鼠脾淋巴细胞与偶联过氧化物酶的抗小鼠Ig孵育，并通过血细胞分析仪进行分析。B淋巴细胞在一个独特的群体中与T淋巴细胞分离，因为它们对过氧化物酶呈阳性。
