Dular U, Chow D A, Paraskevas F
Int J Cancer. 1978 Nov 15;22(5):611-20. doi: 10.1002/ijc.2910220517.
A large dose of tumour cells (10(7)) from two different tumours produced a significant increase of Ig-bearing spleen cells 6 h after intraperitoneal administration in syngeneic mice, like conventional antigens previously tested. Incubation of normal spleen cells in serum taken 6 h after administration of tumour cells reproduced in vitro the changes observed in the spleen cell population of the serum donors, demonstrating the presence of a cytophilic Ig, which was shown to be taken up by T cells. Serum collected 6 h after tumour cell inoculation contains also a 4S factor which can generate in vitro cytophilic Ig for T cells in the presence of a foreign soluble protein or tumour antigen. Extracts obtained from tumours cells, which were shown to contain tumour antigens, were labelled with 125I and mixed with the 4S factor and normal 7S Ig. Upon fractionation of the mixture, high molecular weight material containing radioactivity and 7S Ig were eluted in the void volume, well ahead of the position of both the tumour cell preparation and the 7S Ig. This material contained Ig cytophilic for splenic T cells and it is likely that it represents cytophilic complexes of Ig and tumour antigens. It is postulated that the cytophilic Ig detected in the serum of animals 6 h after injection of a large number of tumour cells represents cytophilic complexes of Ig and tumour antigens formed through the mediation of a soluble factor.
与先前测试的传统抗原一样，给同基因小鼠腹腔注射来自两种不同肿瘤的大剂量肿瘤细胞（10⁷）后6小时，含免疫球蛋白的脾细胞显著增加。用注射肿瘤细胞6小时后采集的血清孵育正常脾细胞，在体外再现了血清供体脾细胞群体中观察到的变化，证明存在一种亲细胞性免疫球蛋白，该免疫球蛋白被T细胞摄取。肿瘤细胞接种6小时后采集的血清中还含有一种4S因子，在存在外来可溶性蛋白质或肿瘤抗原的情况下，该因子可在体外产生针对T细胞的亲细胞性免疫球蛋白。从肿瘤细胞中提取的、已证明含有肿瘤抗原的提取物，用¹²⁵I标记后与4S因子和正常7S免疫球蛋白混合。对混合物进行分级分离时，含有放射性的高分子量物质和7S免疫球蛋白在空体积中被洗脱，远远早于肿瘤细胞制剂和7S免疫球蛋白的位置。该物质含有对脾T细胞有亲细胞性的免疫球蛋白，很可能它代表免疫球蛋白与肿瘤抗原的亲细胞性复合物。据推测，在注射大量肿瘤细胞6小时后动物血清中检测到的亲细胞性免疫球蛋白代表通过可溶性因子介导形成的免疫球蛋白与肿瘤抗原的亲细胞性复合物。
