Vaillier D, Vaillier J
Br J Cancer. 1980 Nov;42(5):722-9. doi: 10.1038/bjc.1980.307.
The mean electrophoretic mobility (EPM) of splenic cells was determined in 4 different host-tumour systems. In splenic cells harvested from mice bearing slow-growing tumours, a significant EPM decrease was observed (12%) and in increase in the proportion of cells with slow mobility. Moreover, after 1 h incubation in RPMI medium at 37 degrees C and 2 washings, spleen cells showed a marked increase in their EPM (average 30%). Finally, the supernatant from incubation medium after contact with normal spleen cells (1 h at 37 degrees C) produced a significant decrease in their EPM (approximately 12%). On the other hand, no significant EPM variations were found between control spleen cells and cells from fast-growing tumours, before or after 1h incubation. The existence of factors which induce a loss of negative surface charge on spleen cells of some tumour-grafted mice is discussed.
在4种不同的宿主-肿瘤系统中测定了脾细胞的平均电泳迁移率（EPM）。在从携带生长缓慢肿瘤的小鼠体内收获的脾细胞中，观察到显著的EPM降低（12%），且迁移缓慢的细胞比例增加。此外，在37℃的RPMI培养基中孵育1小时并洗涤2次后，脾细胞的EPM显著增加（平均30%）。最后，与正常脾细胞接触（37℃ 1小时）后的孵育培养基上清液使其EPM显著降低（约12%）。另一方面，在孵育1小时之前或之后，对照脾细胞与来自生长快速肿瘤的细胞之间未发现显著的EPM变化。本文讨论了某些肿瘤移植小鼠脾细胞表面负电荷丢失诱导因子的存在情况。
