Tobet S A, Chickering T W, Fox T O, Baum M J
Department of Biochemistry and Developmental Neurobiology, Shriver Center, Waltham, Mass 02254.
Neuroendocrinology. 1993 Sep;58(3):316-24. doi: 10.1159/000126556.
Estrogen receptors were visualized in adult ferret brains using the H222 estrogen receptor antibody and immunocytochemical techniques. H222 immunoreactive (H222ir) cell nuclei were present in many forebrain regions in gonadectomized ferrets of both sexes. In many instances, H222ir cells also had immunoreaction product in their processes. All cells with H222ir processes also contained H222ir nuclei. More H222ir processes were observed in females in the medial and lateral preoptic area/anterior hypothalamus, and at the level of the descending fornix and caudal anterior commissure. Quantitative image analysis confirmed that females had significantly more (approximately 50%) extranuclear H222 immunoreaction product than males in cells in the magnocellular or preoptic subnuclei of the bed nucleus of the stria terminalis. Cells in the principal subnucleus of the bed nucleus of the stria terminalis and ventrolateral septum were notable for the relative paucity of H222ir processes. Sex differences in the intracellular extranuclear distribution of estrogen receptor protein in particular brain regions might contribute to the differential regulation of estrogen-dependent functions in the two sexes.
利用H222雌激素受体抗体和免疫细胞化学技术，在成年雪貂大脑中观察到了雌激素受体。在两性去势雪貂的许多前脑区域都存在H222免疫反应性（H222ir）细胞核。在许多情况下，H222ir细胞的突起中也有免疫反应产物。所有具有H222ir突起的细胞也都含有H222ir细胞核。在雌性动物的内侧和外侧视前区/下丘脑前部，以及穹窿降部和尾侧前连合水平，观察到更多的H222ir突起。定量图像分析证实，在终纹床核大细胞或视前亚核的细胞中，雌性动物的核外H222免疫反应产物比雄性动物显著更多（约50%）。终纹床核主亚核和腹外侧隔区的细胞中H222ir突起相对较少。特定脑区雌激素受体蛋白细胞内核外分布的性别差异可能导致两性中雌激素依赖性功能的差异调节。
