在植物线粒体中，一个转运RNA（tRNA）基因转录起始位点类似于信使RNA（mRNA）和核糖体RNA（rRNA）启动子。
A tRNA gene transcription initiation site is similar to mRNA and rRNA promoters in plant mitochondria.
作者信息
Binder S, Brennicke A
机构信息
Institut für Genbiologische Forschung, Berlin, Germany.
出版信息
Nucleic Acids Res. 1993 Nov 11;21(22):5012-9. doi: 10.1093/nar/21.22.5012.
The gene for tRNA(Phe) is located 292 nucleotides upstream of the tRNA(Pro) gene in the Oenothera mitochondrial genome. Hybridization with in vitro capped primary transcripts indicates a transcription initiation site in the 5' region of the gene for tRNA(Phe). Primer extension experiments show the presence of precursor transcripts covering tRNA(Phe) and adjacent sequences up to a transcription initiation site 181 or 180 nucleotides upstream of the tRNA gene. The genomic sequence at this transcription initiation site contains the consensus motif derived for putative promoters of mitochondrial protein and rRNA coding genes in dicotyledonous plants. This sequence similarity suggests that tRNAs, rRNAs and mRNAs can be transcribed from homologous promoters in plant mitochondria.
在月见草属植物线粒体基因组中，tRNA（苯丙氨酸）基因位于tRNA（脯氨酸）基因上游292个核苷酸处。与体外加帽的初级转录本杂交表明，tRNA（苯丙氨酸）基因5'区域存在一个转录起始位点。引物延伸实验表明，存在覆盖tRNA（苯丙氨酸）及其相邻序列直至tRNA基因上游181或180个核苷酸处转录起始位点的前体转录本。该转录起始位点的基因组序列包含双子叶植物线粒体蛋白质和rRNA编码基因推定启动子的共有基序。这种序列相似性表明，tRNA、rRNA和mRNA可以从植物线粒体中的同源启动子转录而来。
Zotero 插件