Binder S, Schuster W, Grienenberger J M, Weil J H, Brennicke A
Institut für Genbiologische Forschung, Berlin.
Curr Genet. 1990 Apr;17(4):353-8. doi: 10.1007/BF00314884.
The genes coding for tRNA(Gly), tRNA(His), tRNA(Lys), tRNA(Phe), tRNA(Ser) and tRNA(Tyr) have been identified in Oenothera mitochondrial DNA. Sequence analysis of these genes and their surrounding sequences are presented and compared with other known tRNA genes from plant mitochondria. All six deduced tRNA sequences can be folded into the classical cloverleaf structure model. Only the tRNA(His) gene shows high homology with the corresponding chloroplast gene and thus appears to be derived from a transfer event of chloroplast sequences into the mitochondrial genome. The sequences surrounding this gene, however, show little similarity with the chloroplast genome. The other five deduced tRNAs display a much lower similarity with their chloroplast counterparts and thus appear to be genuine mitochondrial tRNAs. These tRNAs are highly conserved between monocots and dicots with maximally three nucleotides differing between the Oenothera sequences and their wheat homologues. A purine-rich sequence is found upstream of each tRNA gene in Oenothera, similar to wheat mitochondrial tRNA genes, that could be involved in transcription signalling.
已在月见草属植物线粒体DNA中鉴定出编码tRNA（甘氨酸）、tRNA（组氨酸）、tRNA（赖氨酸）、tRNA（苯丙氨酸）、tRNA（丝氨酸）和tRNA（酪氨酸）的基因。本文给出了这些基因及其周围序列的序列分析结果，并与来自植物线粒体的其他已知tRNA基因进行了比较。所有六个推导的tRNA序列都可以折叠成经典的三叶草结构模型。只有tRNA（组氨酸）基因与相应的叶绿体基因具有高度同源性，因此似乎源自叶绿体序列向线粒体基因组的转移事件。然而，该基因周围的序列与叶绿体基因组几乎没有相似性。其他五个推导的tRNA与其叶绿体对应物的相似性要低得多，因此似乎是真正的线粒体tRNA。这些tRNA在单子叶植物和双子叶植物之间高度保守，月见草属植物序列与其小麦同源序列之间最多有三个核苷酸不同。在月见草属植物的每个tRNA基因上游都发现了一个富含嘌呤的序列，类似于小麦线粒体tRNA基因，可能参与转录信号传导。
