Lythgoe J N
J Physiol. 1976 Oct;262(1):1-13. doi: 10.1113/jphysiol.1976.sp011582.
The iridescent layer in the corneal stroma of the scorpion fish, Taurulus (Cottus) bubalis (Scorpaeniformes), is composed of alternating thin lamellae of normal stroma containing collagen fibrils and lamellae of an amorphous dense-staining material. 2. Iridescence is lost after conventional resin embedding procedures, but is retained after embedding in urea-glutaraldehyde polymer. 3. The retention of iridescence and the absence of gross thickness changes during embedding in urea-glutaraldehyde polymer are an indication that the fine structure of the cornea is less altered than by conventional resin embedding. 4. It is believed that in life the collagen fibrils in the cornea of the scorpion fish and the cornea of the frog and rabbit are slightly larger and much more closely packed than is revealed by conventional resin-embedded sections.
鲉鱼(鲉属,鲉形目)角膜基质中的彩虹色层由交替排列的薄片组成,其中正常基质薄片含有胶原纤维,无定形致密染色物质薄片。2. 经传统树脂包埋程序后彩虹色消失,但在尿素 - 戊二醛聚合物中包埋后仍保留。3. 在尿素 - 戊二醛聚合物中包埋时彩虹色的保留以及总厚度无明显变化表明,角膜的精细结构比传统树脂包埋的改变更小。4. 据信,在活体中,鲉鱼、青蛙和兔子角膜中的胶原纤维比传统树脂包埋切片所显示的略大且排列紧密得多。