Perry L L, Benacerraf B, Greene M I
J Immunol. 1978 Dec;121(6):2144-7.
The nature and function of suppressor factor(s) elaborated by suppressor T cells in response to certain chemically induced tumors have been further defined. Thus, suppressor factor(s) specific for the S1509a methylchol-anthrene-induced fibrosarcoma have been shown to bear determinants encoded by the I-J subregion of the murine MHC since suppressive activity is removed by passage of the factor through an immunoadsorbent composed of anti-I-Jk coupled to Sepharose. No loss of activity was observed after passage of factor through control columns composed of normal mouse globulin. Furthermore, activity could be recovered from the relevant immunoadsorbent by elution with high salt. The administration of crude suppressor factor(s) to normal animals for 4 days resulted in the development of a population of suppressor cells that act in a manner analogous to the suppressor cell population used for production of factor. These factor-induced suppressor cells are T cells and exhibit an antigen specificity similar to that displayed by the tumor-induced suppressor cells. Thus, tumor-specific suppressor factor(s) bear I-J determinants and are capable of inducing the appearance of suppressor T cells in the nontumor-bearing host, which may then act in a specific manner to limit host responsiveness to tumor antigen.
抑制性T细胞针对某些化学诱导肿瘤所产生的抑制因子的性质和功能已得到进一步明确。因此，已证明对S1509a甲基胆蒽诱导的纤维肉瘤具有特异性的抑制因子带有由小鼠MHC的I-J亚区编码的决定簇，因为该因子通过由偶联至琼脂糖的抗I-Jk组成的免疫吸附剂后，抑制活性被消除。该因子通过由正常小鼠球蛋白组成的对照柱后，未观察到活性丧失。此外，通过高盐洗脱可从相关免疫吸附剂中回收活性。将粗制抑制因子给予正常动物4天，导致一群抑制细胞的产生，这些抑制细胞的作用方式类似于用于产生因子的抑制细胞群体。这些因子诱导的抑制细胞是T细胞，并且表现出与肿瘤诱导的抑制细胞相似的抗原特异性。因此，肿瘤特异性抑制因子带有I-J决定簇，并且能够在无肿瘤宿主中诱导抑制性T细胞的出现，然后这些抑制性T细胞可以以特定方式作用以限制宿主对肿瘤抗原的反应性。
