Sehgal S, Aikat B K, Pathania A G
Bull World Health Organ. 1982;60(6):945-50.
Patients with Indian kala-azar were investigated for the presence of circulating immune complexes by the platelet aggregation test, complement deviation test, and polyethylene glycol precipitation test. Circulating antibodies were tested by the conventional indirect immunofluorescence test using leptomonad forms of Leishmania donovani and Crithidia luciliae. The serum complement level (C3) was measured using the Mancini technique. The results indicate that a large number of patients with Indian kala-azar carry circulating immune complexes with a significant lowering of complement levels in their sera. These complexes may be intimately linked with the depressed cell-mediated immune responses that are commonly observed in these patients. The study warrants a detailed immunohistopathological examination of the kidneys for the presence of tissue-bound complexes in chronic patients. Further, it is revealed that Crithidia luciliae and Leishmania donovani share common antigens and the former can be used as a substitute for determining anti-leishmania antibody by the indirect fluorescence assay.
通过血小板聚集试验、补体偏离试验和聚乙二醇沉淀试验，对印度黑热病患者进行循环免疫复合物检测。采用杜氏利什曼原虫和路氏锥虫的前鞭毛体形式，通过传统间接免疫荧光试验检测循环抗体。采用曼奇尼技术测定血清补体水平（C3）。结果表明，大量印度黑热病患者携带循环免疫复合物，其血清补体水平显著降低。这些复合物可能与这些患者中常见的细胞介导免疫反应抑制密切相关。该研究有必要对慢性患者的肾脏进行详细的免疫组织病理学检查，以确定是否存在组织结合复合物。此外，研究发现路氏锥虫和杜氏利什曼原虫具有共同抗原，前者可作为间接荧光法检测抗利什曼原虫抗体的替代物。
