Christoffersen A B
Nord Vet Med. 1976 Nov;28(11):564-9.
The investigation comprised 192-positive and Gram-negative strains of bacteria, including 111 Proteus sp. The degree of resistance of these strains to nalidixic-acid was investigated on blood agar with 25 mug, 40 mug, and 50 mug nalidixin-acid per ml medium. None of the Proteus strains were able to grow on medium with 50 mug nalidixic-acid per ml, whereas Streptococcus sp., Corynebacterium pyogenes, Peptococcus indolicus, Pseudomonas aeruginosa, and beta-toxic staphylococci all were found resistant to this concentration (Tables I and II), and could be reisolated in pure culture after having grown together with a Proteus strain on such medium (Table III). Blood agar with 50 mug nalidixic-acid per ml is therefore suitable for isolation of the above mentioned Gram-positive bacteria and Pseudomonas aeruginosa from Proteus contaminated material.
该研究包括192株革兰氏阳性和阴性细菌菌株，其中包括111株变形杆菌属。在每毫升培养基含25微克、40微克和50微克萘啶酸的血琼脂上研究了这些菌株对萘啶酸的耐药程度。没有一株变形杆菌菌株能够在每毫升含50微克萘啶酸的培养基上生长，而链球菌属、化脓性棒状杆菌、产吲哚消化球菌、铜绿假单胞菌和β-毒素葡萄球菌均对该浓度耐药（表I和表II），并且在与变形杆菌菌株在此类培养基上共同生长后可在纯培养物中再次分离得到（表III）。因此，每毫升含50微克萘啶酸的血琼脂适用于从被变形杆菌污染的材料中分离上述革兰氏阳性细菌和铜绿假单胞菌。
