Matousek J, Rakouský S
Department of Molecular Genetics, Academy of Sciences of the Czech Republik, Ceské Budĕjovice.
Folia Biol (Praha). 1993;39(2):87-99.
The effect of oligodeoxyribonucleotides complementary to the region of the so-called pathogenicity domain (nucleotides 42-78) of the upper RNA strand of potato spindle tuber viroid (PSTVd) (severe) on viroid infection was investigated. The oligonucleotides were allowed to form hybrids with PSTVd in the infection mixtures before inoculation. Infectivity tests were performed using intact plants and plant protoplasts. It was found that the DNA oligonucleotides caused significant reduction of viroid infection at plant and single cell levels. The 200-fold molar excess of antisense DNA over viroid RNA is usually sufficient for the complete blocking of viroid infection. The inhibitory effect is strongly sequence specific. Inhibition by corresponding antisense RNA was much less efficient than that caused by antisense DNA.
研究了与马铃薯纺锤块茎类病毒（PSTVd）（重度）上链RNA所谓致病结构域区域（核苷酸42 - 78）互补的寡脱氧核糖核苷酸对类病毒感染的影响。在接种前，让寡核苷酸在感染混合物中与PSTVd形成杂交体。使用完整植株和植物原生质体进行感染性测试。结果发现，DNA寡核苷酸在植物和单细胞水平上均能显著降低类病毒感染。反义DNA相对于类病毒RNA的摩尔过量200倍通常足以完全阻断类病毒感染。抑制作用具有强烈的序列特异性。相应反义RNA的抑制效率远低于反义DNA。
