Lysenkov N V
Prikl Biokhim Mikrobiol. 1976 Jan-Feb;12(1):24-9.
The activity of carboxy- and aminopeptidases of enzymic preparations obtained from bacteria Bacillus subtilis 1 M, 17, Bacillus mesentericus 100/26, 100/56, 16; Pseudomonas aeruginosa 1/7a, 1/4; Bacillus thermophilicus S. sp. nov B-22, moulds Aspergillus oryzae 1-746, Aspergillus flavus 716 and actinomycete Streptomyces griseus 32-13 was studied. In the bacterial preparations the proteolytic activity was 1.5-2.5 times lower than in the fungal and actinomycete preparations. The carboxypeptidase activity of bacterial preparations was comparable with that of the moulds and by one order of magnitude lower than that of the actinomycete. The aminopeptidase activity of the bacterial preparations was different; however, it was comparable with that of the moulds and was higher than that in some cases. In the Streptomyces preparation the aminopeptidase activity was significantly higher than in the bacterial and fungal preparations. In most preparations Co2+ and Mn2+ influenced the activity of carboxy- and amino-peptidases. This suggests that these proteins can be referred to metal enzymes.
对从枯草芽孢杆菌1M、17，肠系膜芽孢杆菌100/26、100/56、16，铜绿假单胞菌1/7a、1/4，嗜热芽孢杆菌S. sp. nov B - 22，米曲霉1 - 746、黄曲霉716以及灰色链霉菌32 - 13中获得的酶制剂的羧肽酶和氨肽酶活性进行了研究。在细菌酶制剂中，蛋白水解活性比真菌和放线菌酶制剂低1.5 - 2.5倍。细菌酶制剂的羧肽酶活性与霉菌的相当，比放线菌的低一个数量级。细菌酶制剂的氨肽酶活性有所不同；然而，它与霉菌的相当，在某些情况下比霉菌的高。在链霉菌酶制剂中，氨肽酶活性显著高于细菌和真菌酶制剂。在大多数酶制剂中，Co2 +和Mn2 +影响羧肽酶和氨肽酶的活性。这表明这些蛋白质可归为金属酶。
