Stoll K E, Duncan J R
Department of Biochemistry and Microbiology, Rhodes University, Grahamstown, South Africa.
Prostaglandins Leukot Essent Fatty Acids. 1993 Oct;49(4):771-6. doi: 10.1016/0952-3278(93)90024-q.
Ascorbate has been shown to be involved in essential fatty acid (EFA) metabolism, resulting in the suggestion that the effect of ascorbate on cell growth may be mediated through an influence on the metabolism of these FAs. This study examined the effect of ascorbate, supplemented over the nutritional concentration range of 0-100 micrograms/ml, on the in vitro cell growth of non-malignant LLCMK (monkey kidney) cells and malignant B16 murine melanoma cells. The effect of ascorbate on EFA composition was also investigated, and involved the determination of the levels of linoleic acid (LA), gamma-linoleic acid (GLA), dihomogammalinolenic acid (DGLA) and arachidonic acid (AA) present in the stroma and membrane of the two cell types. Ascorbate had no significant inhibitory or stimulatory effect on the growth of either the LLCMK or B16 cells. EFA levels detected in the LLCMK cells were generally higher than those detected in the B16 cells. The % composition of the various EFAs in the stroma fractions of the two cell types were higher than the level of the corresponding EFAs in the membrane fractions. GLA levels were not detectable in the membrane fractions of the B16 cells. AA % composition determined in both cell types, was greater than that of any other EFA % composition.
已证明抗坏血酸参与必需脂肪酸（EFA）代谢，这表明抗坏血酸对细胞生长的影响可能是通过影响这些脂肪酸的代谢来介导的。本研究考察了在0 - 100微克/毫升营养浓度范围内添加抗坏血酸对非恶性LLCMK（猴肾）细胞和恶性B16小鼠黑色素瘤细胞体外细胞生长的影响。还研究了抗坏血酸对必需脂肪酸组成的影响，包括测定两种细胞类型的基质和膜中存在的亚油酸（LA）、γ-亚油酸（GLA）、二高γ-亚麻酸（DGLA）和花生四烯酸（AA）的水平。抗坏血酸对LLCMK或B16细胞的生长均无显著抑制或刺激作用。在LLCMK细胞中检测到的必需脂肪酸水平通常高于在B16细胞中检测到的水平。两种细胞类型的基质部分中各种必需脂肪酸的百分比组成高于膜部分中相应必需脂肪酸的水平。在B16细胞的膜部分中未检测到GLA水平。在两种细胞类型中测定的AA百分比组成均大于任何其他必需脂肪酸的百分比组成。
