体外培养细胞中的磷脂酶A2活性——抗坏血酸补充的影响
Phospholipase A2 activity in vitro cultured cells--influence of ascorbate supplementation.
作者信息
Stoll K E, Duncan J R
机构信息
Department of Biochemistry and Microbiology, Rhodes University, Grahamstown, South Africa.
出版信息
J Lipid Mediat Cell Signal. 1995 May;11(3):261-6. doi: 10.1016/0929-7855(94)00043-c.
Non-malignant LLCMK cells and malignant B16 cells were supplemented with ascorbate, over the concentration range 0-100 micrograms/ml ascorbate. The effects of ascorbate supplementation on cell growth and phospholipase A2 activity of the membrane fractions of the respective cell lines were determined. Increasing ascorbate supplementation had a significant inhibitory effect on the growth of the B16 cells. Phospholipase A2 activity in the control B16 cells was lower than that detected in the control LLCMK cells. Phospholipase A2 activity decreased significantly in the B16 cells upon increasing ascorbate supplementation, while the supplementation of ascorbate to the LLCMK cells did not have any significant effect on phospholipase A2 activity in these cells.
非恶性的LLCMK细胞和恶性的B16细胞分别添加了抗坏血酸，抗坏血酸的浓度范围为0至100微克/毫升。测定了添加抗坏血酸对相应细胞系细胞膜组分的细胞生长和磷脂酶A2活性的影响。增加抗坏血酸的添加量对B16细胞的生长具有显著的抑制作用。对照B16细胞中的磷脂酶A2活性低于对照LLCMK细胞中检测到的活性。随着抗坏血酸添加量的增加，B16细胞中的磷脂酶A2活性显著降低，而向LLCMK细胞中添加抗坏血酸对这些细胞中的磷脂酶A2活性没有任何显著影响。
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