Angus R, Collins C M, Symes M O
University Department of Surgery, Bristol Royal Infirmary, U.K.
Eur J Cancer. 1993;29A(13):1879-85. doi: 10.1016/0959-8049(93)90542-n.
Tumour cells were separated from 19 renal carcinomas and cultured in vitro. The effect of interferon (IFN) alpha and gamma on cell proliferation was measured and compared to the effect of IFN on the expression of class I and class II major histocompatibility complex (MHC) antigens. When tested within the first 14 days of culture, IFN-alpha inhibited protein synthesis in 12 of 15 and IFN-gamma in four of nine tumours. Reduction in cell counts was in parallel. In six tumours the culture period was extended and in all six the effect of IFN-alpha was lost. Exposure to IFN-alpha induced or enhanced class I antigen expression in eight of 19 tumours and class II expression in two of 19. The analogous figures for IFN-gamma were five and three tumours. In four of five cases where a comparison could be made there was a correlation between the effects of IFN-alpha on cell proliferation and class I antigen expression. The efficacy of IFN in the treatment of renal carcinomas may thus, in part, result from inhibition of cell proliferation and enhancement of antigen expression.
从19例肾癌中分离出肿瘤细胞并进行体外培养。测定了α干扰素（IFN）和γ干扰素对细胞增殖的影响，并与IFN对I类和II类主要组织相容性复合体（MHC）抗原表达的影响进行了比较。在培养的前14天内进行检测时，α干扰素抑制了15例中的12例肿瘤细胞的蛋白质合成，γ干扰素抑制了9例中的4例。细胞计数的减少与之平行。在6例肿瘤中延长了培养期，在所有6例中α干扰素的作用消失。暴露于α干扰素可诱导或增强19例中的8例肿瘤的I类抗原表达以及19例中的2例肿瘤的II类抗原表达。γ干扰素的相应数字分别为5例和3例肿瘤。在5例可进行比较的病例中，有4例α干扰素对细胞增殖的作用与I类抗原表达之间存在相关性。因此，IFN治疗肾癌的疗效可能部分源于对细胞增殖的抑制和抗原表达的增强。
