Kuzemenská P, Burian V, Nováková E
Zentralbl Bakteriol Orig A. 1976 Oct;236(1):16-21.
By means of a model experiment the possibility of preserving a N. meningitidis strain by freezing was demonstrated and verified. The results of cultivating the strain after storage at - 20 degrees C, - 15 degrees C and +4 degrees C for 4, 6, 24 and 48 hours were compared. The temperature of - 20 degrees C was found to be the most reliable one for preserving the viability of the N. meningitidis strain. The method of freezing the strain is of practical importance under conditions where no low-freezing box or liquid nitrogen are available.
通过模型实验证明并验证了通过冷冻保存脑膜炎奈瑟菌菌株的可能性。比较了该菌株在-20℃、-15℃和+4℃下储存4、6、24和48小时后培养的结果。发现-20℃是保存脑膜炎奈瑟菌菌株活力最可靠的温度。在没有低温冰箱或液氮的条件下，冷冻菌株的方法具有实际意义。
