Amesse Lawrence S, Srivastava Geeta, Uddin David, Pfaff-Amesse Teresa
Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Wright State University School of Medicine, 128 Apple Street, Dayton, OH 45409, USA.
J Reprod Med. 2003 May;48(5):319-24.
To evaluate the feasibility of using the vaporous phase of liquid nitrogen as a storage medium for human semen samples.
Human semen samples were used in a 2-armed study. In the first arm, semen samples were rapidly frozen and randomly assigned to storage in either liquid nitrogen (-196 degrees C) or vapor-phase nitrogen (-189 degrees C). In the second arm, sperm specimens were frozen in a controlled, stepwise process and randomly assigned to storage in either the vaporous or liquid phase of liquid nitrogen. The samples were subsequently thawed and analyzed for sperm viability.
The rapid freezing technique demonstrated no differences in the examined viability parameters between semen specimens stored in either liquid or vaporous nitrogen. Similarly, semen samples prepared by the slower, controlled freezing process also showed no differences in viability parameters after storage in either the liquid or vaporous phase of liquid nitrogen. The rapid freezing technique was equal to the slow, controlled freezing method in terms of preserving the integrity of the sperm when the specimens were subsequently stored in either liquid or vaporous nitrogen.
Cryopreservation of human semen in the vapor phase of nitrogen is a viable alternative to storage in liquid-phase nitrogen.
评估使用液氮气相作为人类精液样本储存介质的可行性。
在一项双臂研究中使用人类精液样本。在第一组中，精液样本被快速冷冻并随机分配至液氮（-196℃）或气相氮（-189℃）中储存。在第二组中，精子标本以可控的逐步过程冷冻，并随机分配至液氮的气相或液相中储存。随后将样本解冻并分析精子活力。
快速冷冻技术显示，储存在液氮或气相氮中的精液标本在所检测的活力参数方面没有差异。同样，通过较慢的可控冷冻过程制备的精液样本在储存在液氮的液相或气相中后，活力参数也没有差异。当标本随后储存在液氮或气相氮中时，就保持精子完整性而言，快速冷冻技术与缓慢的可控冷冻方法相当。
在氮的气相中冷冻保存人类精液是液相氮储存的可行替代方法。
