Cludts I, Cleuter Y, Kettmann R, Burny A, Droogmans L
Department of Molecular Biology, University of Brussels (ULB), Belgium.
Cytokine. 1993 Jul;5(4):336-41. doi: 10.1016/1043-4666(93)90065-d.
The screening of a bovine genomic library with a human tumour necrosis factor-alpha (TNF-alpha) cDNA probe resulted in the isolation of a 7.2 kb DNA fragment containing the entire bovine TNF-alpha gene. Analysis of this genomic clone showed that it also contains the bovine lymphotoxin (LT, TNF-beta) gene. Comparison to published sequences of human, murine, ovine and rabbit counterparts allowed us to delineate the coding sequences, the promoters and the enhancers of these two genes. Sequences involved in the regulation of translation and in the mRNA stability were found in the 3' untranslated regions.
用人肿瘤坏死因子-α（TNF-α）cDNA探针筛选牛基因组文库，得到一个7.2 kb的DNA片段，其中包含完整的牛TNF-α基因。对该基因组克隆的分析表明，它还包含牛淋巴毒素（LT，TNF-β）基因。与已发表的人、小鼠、绵羊和兔对应基因序列进行比较，使我们能够确定这两个基因的编码序列、启动子和增强子。在3'非翻译区发现了参与翻译调控和mRNA稳定性的序列。
