Monteyne P, Van Broeck J, Van Snick J, Coutelier J P
Unit of Experimental Medicine, Université Catholique de Louvain, Brussels, Belgium.
Cytokine. 1993 Jul;5(4):394-7. doi: 10.1016/1043-4666(93)90073-e.
Interleukin 4 (IL-4) and gamma interferon (IFN-gamma) gene expression in lymph node cells from mice immunized with keyhole limpet haemocyanin in the presence of complete Freund's adjuvant was analysed by polymerase chain reaction. This immunization clearly induced the expression of IL-4 message, whereas IFN-gamma message was only slightly increased. A concomitant infection with lactate dehydrogenase-elevating virus drastically decreased IL-4 expression whereas IFN-gamma message was not modified. Analysis of the kinetics of cytokine production indicated that the virus did not induce a mere change in the timing of lymphokine production, but a persistent inhibition of IL-4.
用钥孔戚血蓝蛋白在完全弗氏佐剂存在的情况下免疫小鼠，通过聚合酶链反应分析来自这些小鼠淋巴结细胞中的白细胞介素4（IL-4）和γ干扰素（IFN-γ）基因表达。这种免疫明显诱导了IL-4信使的表达，而IFN-γ信使只是略有增加。同时感染乳酸脱氢酶升高病毒会显著降低IL-4的表达，而IFN-γ信使未发生改变。细胞因子产生动力学分析表明，该病毒并非仅仅诱导淋巴因子产生时间的改变，而是对IL-4产生持续抑制。
