Wilhelm K S, Thomashow M F
Department of Crop and Soil Sciences, Michigan State University, East Lansing 48824.
Plant Mol Biol. 1993 Dec;23(5):1073-7. doi: 10.1007/BF00021822.
We previously described a cold-regulated (cor) gene from Arabidopsis thaliana, cor15a (formerly cor15), that encodes a 15 kDa polypeptide that is targeted to chloroplasts (C. Lin and M. Thomashow, Plant Physiol 99 (1992): 519-525). Here we describe an apparent homologue of cor15a, cor15b. cor15b is located immediately downstream from cor15a (within 1 kb) and has the same relative 5' to 3' orientation as cor15a. The predicted coding regions for the two genes are 82% identical at the nucleic acid sequence level. Transcripts for cor15b, like those of cor15a, increase dramatically in response to low temperature and exogenous application of ABA. However, only cor15a transcripts accumulate to high levels in response to drought. The cor15a/cor15b gene pair is the third case in which A. thaliana cold-regulated genes have been found to exist as tandem gene pairs with the members of each pair being differentially regulated.
我们之前描述过拟南芥中的一个冷调节（cor）基因cor15a（以前称为cor15），它编码一种15 kDa的多肽，该多肽定位于叶绿体（C. Lin和M. Thomashow，《植物生理学》99（1992）：519 - 525）。在此我们描述了cor15a的一个明显同源物cor15b。cor15b位于cor15a紧下游（在1 kb范围内），并且与cor15a具有相同的从5'到3'的相对方向。这两个基因的预测编码区在核酸序列水平上有82%的同一性。cor15b的转录本，与cor15a的转录本一样，在低温和外源施加脱落酸（ABA）时显著增加。然而，只有cor15a的转录本在干旱时积累到高水平。cor15a/cor15b基因对是拟南芥冷调节基因以串联基因对形式存在的第三个例子，其中每对成员受到不同的调节。
