Joshi C P
Nucleic Acids Res. 1987 Aug 25;15(16):6643-53. doi: 10.1093/nar/15.16.6643.
Over 75 published genomic DNA sequences from several higher plants have been collected and flanking regions of the leader sequences have been analysed. In a majority of the plants, the first AUG codon on processed mRNA acted as a translation initiation site. The consensus sequence for the context was TAAACAATGGCT (on plus strand of DNA). This differed from the earlier suggestion for eukaryotic mRNAs based mainly on data from animals. Leader sequences were generally 40-80 nucleotides in length and were A+T rich. Adenine was present in a majority of the cases at the transcription start site which was flanked by pyrimidine bases. The putative TATA box was present 32 +/- 7 nucleotides upstream from the transcription initiation site. The consensus sequence for TATA box and surrounding region was TCACTATATATAG.
已收集了来自几种高等植物的75多个已发表的基因组DNA序列,并对前导序列的侧翼区域进行了分析。在大多数植物中,加工后的mRNA上的第一个AUG密码子充当翻译起始位点。上下文的共有序列为TAAACAATGGCT(在DNA的正链上)。这与早期主要基于动物数据对真核mRNA的建议不同。前导序列通常长度为40-80个核苷酸,且富含A+T。在大多数情况下,腺嘌呤存在于转录起始位点,该位点两侧为嘧啶碱基。假定的TATA框位于转录起始位点上游32 +/- 7个核苷酸处。TATA框及其周围区域的共有序列为TCACTATATATAG。