Taliani G, Badolato M C, Lecce R, Bruni R, Clementi C, Grimaldi F, Furlan C, Manganaro M, Duca F, Bozza A
Institute of Tropical Disease, La Sapienza University, Policlinico Umberto I, Rome, Italy.
Arch Virol Suppl. 1993;8:219-28. doi: 10.1007/978-3-7091-9312-9_22.
The presence of circulating hepatitis C virus genome (HCV-RNA), elevated ALT levels and antibodies to an NS5-derived synthetic peptide have been examined in 13 subjects with isolate positivity for antibodies to the HCV core antigen (C22) on RIBA-2 testing. All subjects were followed up for 8-18 months (mean 12.4 months). In seven subjects (54%), intermittent or persistent viremia was associated with abnormal ALT levels (6 subjects) and with positivity for antibodies to NS5-peptide (6 subjects). On the other hand, in 6 out of 13 subjects (46%) no viral replication, no liver cytonecrosis and no antibodies to NS5 were found. It is concluded that isolate reactivity to C22 by RIBA-2 is a heterogeneous condition that corresponds to two distinct categories of subjects: those with active HCV infection and those without evidence of virus replication. Although HCV-RNA determination is the most reliable means of identifying HCV carriers, antibodies to NS5 can be a useful marker of virus activity. In fact, antibodies to NS5 were detected in 6 out of 7 viremic patients, compared to 0 out of 6 non-viremic patients (P = 0.004). It remains to be elucidated whether the isolate reactivity to core antigen found in non-viremic subjects represents a specific, HCV-induced antibody response, or is an unrelated crossreactivity.
对13名在重组免疫印迹法-2（RIBA-2）检测中丙肝病毒核心抗原（C22）抗体呈单独阳性的受试者，检测了循环丙肝病毒基因组（HCV-RNA）的存在情况、丙氨酸转氨酶（ALT）水平升高情况以及针对NS5衍生合成肽的抗体。所有受试者均随访了8 - 18个月（平均12.4个月）。7名受试者（54%）中，间歇性或持续性病毒血症与ALT水平异常（6名受试者）以及NS5肽抗体阳性（6名受试者）相关。另一方面，13名受试者中有6名（46%）未发现病毒复制、肝细胞坏死以及NS5抗体。得出的结论是，RIBA-2检测中对C22的单独反应性是一种异质性情况，对应两类不同的受试者：有活动性丙肝病毒感染的受试者和无病毒复制证据的受试者。虽然HCV-RNA测定是识别丙肝病毒携带者最可靠的方法，但NS5抗体可能是病毒活性的一个有用标志物。事实上，7名病毒血症患者中有6名检测到NS5抗体，相比之下，6名非病毒血症患者中无一例检测到（P = 0.004）。非病毒血症受试者中发现的对核心抗原的单独反应性是代表一种特异性的、丙肝病毒诱导的抗体反应，还是一种无关的交叉反应，仍有待阐明。
