Schneider B
Acta Histochem. 1976;56(2):222-44.
The behaviour of several dehydrogenases(succino-, beta-glycerophosphate-, lactate-, alcohol-, beta-hydroxibutyric acid-, glucose-6-phosphate-, isocitronic acid-dehydrogenase, monoamino-oxidase, and gamma-aminobutyric acid-transaminase) and of several hydrolytic enzymes (non-specific esterase, lipase, acetylcholin-, butyrylcholinesterase, alkaline phosphatase and leucinaminopeptidase) was investigated in the neurons of NSO and NPV, in the infundibulum and in the neurohypophysis and the innervation of the neurons (acetylcholinesterase, monoamino-oxidase, catecholamines) by unmilked and milked cows. The milking stimulus influences the metabolism especially in the neurosecretory cells of the NPV. After the milking stimulus the activity of oxydative enzymes is above all very increased, the anaerobic way of the output of energy is after that also higher. The building up of the carbohydrates through glycolyse in the neurosecretory cells of the NPV after the milking stimulus is increased. The possible participation of the investigated hydrolytic enzymes on the metabolism of the neurosecretory cells is discussed. The neurons of the NPV were innervated for the most part adrenergic. It is discussed the participation of the enzymes succinodehydrogenase and monoaminooxidase on the hormone release in the neurohypophysis.
研究了未挤奶和已挤奶牛的视上核（NSO）和室旁核（NPV）神经元、漏斗部、神经垂体中几种脱氢酶（琥珀酸脱氢酶、β-甘油磷酸脱氢酶、乳酸脱氢酶、乙醇脱氢酶、β-羟基丁酸脱氢酶、葡萄糖-6-磷酸脱氢酶、异柠檬酸脱氢酶、单胺氧化酶和γ-氨基丁酸转氨酶）以及几种水解酶（非特异性酯酶、脂肪酶、乙酰胆碱酯酶、丁酰胆碱酯酶、碱性磷酸酶和亮氨酰氨肽酶）的活性，以及神经元的神经支配（乙酰胆碱酯酶、单胺氧化酶、儿茶酚胺）情况。挤奶刺激尤其会影响NPV神经分泌细胞的代谢。挤奶刺激后，氧化酶的活性首先会大幅增加，之后能量输出的无氧途径也会更高。挤奶刺激后，NPV神经分泌细胞中通过糖酵解生成碳水化合物的过程增强。讨论了所研究的水解酶可能参与神经分泌细胞代谢的情况。NPV的神经元大部分由肾上腺素能神经支配。还讨论了琥珀酸脱氢酶和单胺氧化酶在神经垂体激素释放中的作用。
