Baytner S, Mitmaker B, Gordon P H, Wang E
Department of Surgery, Bloomfield Centre for Research in Aging, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis--Jewish General Hospital, Montreal, Quebec.
Clin Invest Med. 1993 Oct;16(5):379-85.
The purpose of this study was to analyze the expression of a mutant (MUT) p53 oncogene protein in the mucosal crypts adjacent to a human colon cancer. Five 1-cm mucosal segments were taken from the surgical specimens over a 5-cm distance from the tumor. Immunohistochemistry was performed using a monoclonal antibody (Ab3) to the MUT p53 and examination by light microscopy. The mean % labelling index (LI) of 10 crypts/cm segment was determined by image analysis. The LI for the entire crypt length for the first cm segment was 33.51 +/- 4.2 and for the second cm segment was 29.26 +/- 5.4 (p < 0.02). Due to the unequal distribution of the label within the crypt length, it was divided into halves so that the LI of these levels could be determined. The LI for the upper and lower crypt levels for the first cm segment were 28.67 +/- 3.2 and 78.23 +/- 4.6 (p < 0.01); for the second segment, the LI were 22.0 +/- 5.1 and 68.66 +/- 4.7 (p < 0.01). No expression of MUT p53 nuclear protein was noted distally at 3-5 cm. The localization of MUT p53 protein product to the crypt stem cell nucleus supports the contention that a malignant field change exists in the transitional mucosa adjacent to a human colon cancer.
本研究的目的是分析突变型（MUT）p53癌基因蛋白在人结肠癌旁黏膜隐窝中的表达。从手术标本中在距肿瘤5厘米的距离内取5个1厘米长的黏膜段。使用针对MUT p53的单克隆抗体（Ab3）进行免疫组织化学检测，并通过光学显微镜检查。通过图像分析确定每厘米段10个隐窝的平均标记指数（LI）。第一段1厘米隐窝全长的LI为33.51±4.2，第二段1厘米隐窝全长的LI为29.26±5.4（p<0.02）。由于标记在隐窝长度内分布不均，将其分为两半，以便确定这些水平的LI。第一段1厘米隐窝上半段和下半段的LI分别为28.67±3.2和78.23±4.6（p<0.01）；第二段的LI分别为22.0±5.1和68.66±4.7（p<0.01）。在3 - 5厘米远侧未观察到MUT p53核蛋白的表达。MUT p53蛋白产物定位于隐窝干细胞核，支持了在人结肠癌旁过渡黏膜中存在恶性场改变的观点。
