Tan E M, Hoffren J, Rouda S, Greenbaum S, Fox J W, Moore J H, Dodge G R
Department of Pathology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Exp Cell Res. 1993 Dec;209(2):200-7. doi: 10.1006/excr.1993.1302.
Basic fibroblast growth factor (bFGF) is a multipotential heparin-binding factor that belongs to the fibroblast growth factor (FGF) family. The FGFs demonstrate a wide spectrum of biologic activities in vivo and in vitro. In this study, we investigated the potential of bFGF to regulate the expression of various dermal extracellular matrix proteoglycans and type I collagen mRNAs in cultured human fibroblasts from keloid, which is a prototype of dermal fibrosis, and normal skin tissue. We report that bFGF upregulates the expression of the decorin gene in normal and keloid fibroblasts. In contrast, the expression of biglycan is downregulated by bFGF. The mRNA steady-state level of versican, a large proteoglycan, is not altered by bFGF. Type I collagen gene expression is downregulated substantially in keloid and normal fibroblasts by bFGF. The results suggest that the expression of the proteoglycan genes are uncoordinately regulated and that the gene expression of type I collagen and biglycan is coordinately downregulated. The results also demonstrate that keloid fibroblasts respond similarly as do normal fibroblasts to bFGF in the regulation of proteoglycan and collagen expression.
碱性成纤维细胞生长因子（bFGF）是一种多潜能肝素结合因子，属于成纤维细胞生长因子（FGF）家族。FGFs在体内和体外均表现出广泛的生物学活性。在本研究中，我们研究了bFGF调节瘢痕疙瘩（一种皮肤纤维化的原型）和正常皮肤组织来源的培养人成纤维细胞中各种真皮细胞外基质蛋白聚糖和I型胶原mRNA表达的潜力。我们报道bFGF上调正常和瘢痕疙瘩成纤维细胞中核心蛋白聚糖基因的表达。相反，双糖链蛋白聚糖的表达被bFGF下调。多功能蛋白聚糖versican的mRNA稳态水平不受bFGF影响。I型胶原基因表达在瘢痕疙瘩和正常成纤维细胞中均被bFGF显著下调。结果表明蛋白聚糖基因的表达受到不协调调节，I型胶原和双糖链蛋白聚糖的基因表达受到协同下调。结果还表明，在蛋白聚糖和胶原表达的调节方面，瘢痕疙瘩成纤维细胞对bFGF的反应与正常成纤维细胞相似。
