Ashcroft G S, Horan M A, Ferguson M W
School of Biological Sciences, University of Manchester, UK.
J Anat. 1997 Apr;190 ( Pt 3)(Pt 3):351-65. doi: 10.1046/j.1469-7580.1997.19030351.x.
A number of reports suggest that the process of ageing impairs wound repair and that strategies to manipulate the age-related wound healing environment are necessary in order to stimulate repair. The process of cutaneous wound repair is controlled by growth factors in an autocrine and paracrine fashion: it is therefore surprising that the localisation of specific growth factors and their receptors has not been documented in wound healing with respect to chronological age. In this study the temporal profile of growth factor and receptor immunostaining was assessed within acute incisional wounds in an ageing mouse colony. A delay in appearance of platelet derived growth factor (PDGF) A and B isoforms, and PDGF-alpha and -beta receptors was evident with increasing animal age, paralleled by a similar finding for epidermal growth factor (EGF) and EGF receptor. Transforming growth factor (TGF)-beta 1 and 2 isoforms were increased at all time points in the wounds of younger animals, but the TGF-beta 3 isoform increased in intensity from d 7 postwounding in the old mice wounds, and basic fibroblast growth factor (bFGF) from d 14. The quantity and distribution patterns of the various growth factors and their receptors may explain the age-related differences in wound healing speed and quality, and possibly suggest new therapeutic targets for manipulating wound healing in the aged.
许多报告表明，衰老过程会损害伤口修复，因此有必要采取策略来调控与年龄相关的伤口愈合环境，以促进修复。皮肤伤口修复过程由生长因子以自分泌和旁分泌方式控制：因此，令人惊讶的是，关于按实足年龄划分的伤口愈合过程中，特定生长因子及其受体的定位尚未见文献记载。在本研究中，评估了衰老小鼠群体急性切口伤口内生长因子和受体免疫染色的时间变化情况。随着动物年龄增长，血小板衍生生长因子（PDGF）A和B亚型以及PDGF-α和-β受体的出现明显延迟，表皮生长因子（EGF）和EGF受体也有类似发现。转化生长因子（TGF）-β1和2亚型在年轻动物伤口的所有时间点均增加，但TGF-β3亚型在老年小鼠伤口受伤后第7天强度增加，碱性成纤维细胞生长因子（bFGF）在第14天增加。各种生长因子及其受体的数量和分布模式可能解释了与年龄相关的伤口愈合速度和质量差异，并可能为调控老年伤口愈合提示新的治疗靶点。
