Melik-Sarkisian S S, Bashirova N F, Zaurolova N O, Kretovich V L
Biokhimiia. 1976;41(7):1330-3.
During incubation in the buffer bacteroids Rizobium lupini liberate an enzymic system (ferri-Lb-reductase) capable of reducing ferri-Lb and ferri-Mb The hemoproteids reduction required the presence of cofactors (NADPH or NADH). The ferri-Lb-reductase activity was also found in the soluble fraction of nodules. It is assumed that bacteroids ferri-Lb-reductase, maintains Lb in a physiologically active reduced state into vegetable cells of the nodules "in vivo" as well.
在缓冲液中培养期间,羽扇豆根瘤菌释放出一种能够还原高铁豆血红蛋白(ferri-Lb)和高铁肌红蛋白(ferri-Mb)的酶系统(高铁豆血红蛋白还原酶)。血红素蛋白的还原需要辅因子(NADPH或NADH)的存在。在根瘤的可溶部分也发现了高铁豆血红蛋白还原酶活性。据推测,根瘤菌的高铁豆血红蛋白还原酶在“体内”也能将豆血红蛋白维持在生理活性还原状态,存在于根瘤植物细胞中。