钙和A23187对镰状细胞形状的稳定作用。
Stabilization of the shape of sickled cells by calcium and A23187.
作者信息
Clark M R, Greenquist A C, Shohet S B
出版信息
Blood. 1976 Dec;48(6):899-909.
Evaluation of the role of calcium in irreversible sickling has been approached by treating sickled cells with calcium and the ionophore A23187. A calcium-dependent stabilization of the sickled cell shape was observed after reoxygenation of cells in the presence of ionophore. At low calcium concentrations, this retention of sickled shape was maintained for periods up to 1 hr. However, the morphology of the oxygen-stable sickled cells was like that of deoxygenated sickle cells and significantly different from the characteristic morphology of native irreversibly sickled cells (ISCs). Because the stabilized cells did not fulfill the morphological criterion for ISCs, the shape-stabilizing effect of calcium in this system did not provide additional support for the hypothesis that calcium accumulation was the determining factor in ISC generation.
通过用钙和离子载体A23187处理镰状细胞，对钙在不可逆镰变中的作用进行了评估。在离子载体存在的情况下，细胞复氧后观察到镰状细胞形状的钙依赖性稳定。在低钙浓度下，这种镰状形状的保持可持续长达1小时。然而，氧稳定的镰状细胞的形态与脱氧镰状细胞相似，与天然不可逆镰状细胞（ISC）的特征形态有显著差异。由于稳定化的细胞不符合ISC的形态学标准，因此该系统中钙的形状稳定作用并未为钙积累是ISC产生的决定因素这一假说提供额外支持。
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