Eaton J W, Berger E, White J G, Jacob H S
Br J Haematol. 1978 Jan;38(1):57-62. doi: 10.1111/j.1365-2141.1978.tb07108.x.
An ionophore specific for divalent cations has been used to load normal erythrocytes and erythrocytes from patients with sickle cell anaemia (Hb SS disease), with small amounts of calcium. Such calcium accumulation leads to decreased cellular water, potassium, adenosine triphosphate (ATP), and osmotic fragility, all characteristics of irreversibly sickled cells (ISCs). In addition, calcium loading of Hb SS, but not normal, erythrocytes causes a marked decrease of haemoglobin oxygen affinity; another, and specific, hallmark of ISCs. Ionophore-induced accumulation of calcium by deoxygenated Hb SS erythrocytes also leads to temporary retention of sickled shape following reoxygenation, despite the absence of detectable intracellular haemoglobin S fibres. All these effects require calcium in the incubation medium and support the idea that increased intracellular calcium is important in the formation of ISCs in patients with Hb SS disease.
一种对二价阳离子具有特异性的离子载体已被用于将正常红细胞以及镰状细胞贫血（血红蛋白SS病）患者的红细胞装载少量钙。这种钙积累会导致细胞内水分、钾、三磷酸腺苷（ATP）减少以及渗透脆性降低，这些都是不可逆镰状细胞（ISC）的特征。此外，血红蛋白SS（而非正常）红细胞的钙装载会导致血红蛋白氧亲和力显著降低，这是ISC的另一个特定标志。尽管未检测到细胞内血红蛋白S纤维，但离子载体诱导脱氧的血红蛋白SS红细胞积累钙也会导致复氧后镰状形态的暂时保留。所有这些效应都需要培养基中的钙，并支持细胞内钙增加在血红蛋白SS病患者ISC形成中起重要作用这一观点。
