Haug E, Gautvik K M
Endocrinology. 1976 Dec;99(6):1482-9. doi: 10.1210/endo-99-6-1482.
A clonal strain of rat pituitary tumor cells (GH3) was used to study the effects of different sex steroids on the production of prolactin (PRL). Hormone production was measured by radioimmunoassay and expressed as the amount of hormone which accumulated in the medium of monolayer cultures during 24 h. The stimulatory effect of 17beta-estradiol (10(-11)M-10(-6)M) on PRL production was significant after 4 days and the maximum effect (300% of control cultures) was observed at 10(-8)M after 10 days of treatment. After removal of added 17beta-estradiol, the production of PRL returned to control levels in 5 days. Progesterone (10(-11)M-10(-6)M) caused a dose-related decrease in PRL production reaching 60% of control values at 10(-6)M. Testerone (10(-6)M) stimulated the production of PRL (130% of controls), whereas 5alpha-dihydrotestosterone (10(-6)M) had a small effect (107% of controls) which was not always reproducible. None of the sex steroids affected cell growth. Progesterone (10(-6)M) inhibited the stimulatory effect of 17beta-estradiol (10(-8)M) on PRL production. The effect of 17beta-estradiol (10(-8)M) and thyrotropin releasing hormone (TRH) (3 X 10(-7) M) was addititive, while no additional stimulatory effect was observed when 17beta-estradiol (10(-8)M) was combined with testosterone (10(-6)M). If the properties of the GH3 cells are analogous to those of normal lactotropes, the sex steroids may alter PRL production at the pituitary level, an influence that may be further modulated by TRH.
采用大鼠垂体瘤细胞系（GH3）的一个克隆株来研究不同性类固醇对催乳素（PRL）分泌的影响。通过放射免疫分析法测定激素分泌量，并表示为单层培养物培养基中24小时内积累的激素量。17β-雌二醇（10⁻¹¹M - 10⁻⁶M）对PRL分泌的刺激作用在4天后显著，处理10天后在10⁻⁸M时观察到最大效应（对照培养物的300%）。去除添加的17β-雌二醇后，PRL分泌在5天内恢复到对照水平。孕酮（10⁻¹¹M - 10⁻⁶M）导致PRL分泌呈剂量依赖性下降，在10⁻⁶M时降至对照值的60%。睾酮（10⁻⁶M）刺激PRL分泌（对照的130%），而5α-二氢睾酮（10⁻⁶M）的作用较小（对照的107%）且并非总能重复出现。没有一种性类固醇影响细胞生长。孕酮（10⁻⁶M）抑制17β-雌二醇（10⁻⁸M）对PRL分泌的刺激作用。17β-雌二醇（10⁻⁸M）和促甲状腺激素释放激素（TRH）（3×10⁻⁷M）的作用是相加的，而当17β-雌二醇（10⁻⁸M）与睾酮（10⁻⁶M）联合使用时未观察到额外的刺激作用。如果GH3细胞的特性与正常催乳细胞类似，性类固醇可能在垂体水平改变PRL分泌，这种影响可能会被TRH进一步调节。
