[使用新开发的“SD - 8729”免疫放射分析试剂盒测量CA125的基础研究]
[Basic study of CA125 measurement using a newly developed "SD-8729" IRMA kit].
作者信息
Yamaguchi A, Kobayashi M, Imai T, Otake H, Matsubara K, Hatori N, Tomiyoshi K, Inoue T, Endo K
机构信息
Department of Nuclear Medicine, School of Medicine, Gunma University.
出版信息
Kaku Igaku. 1993 Oct;30(10):1245-52.
"SD-8729" is a one-step IRMA kit employing OC125 antibody as 125I-labeled tracer and M-11 antibody as an immunoadsorbent. Higher bound-radioactivity to beads was observed with shorter incubation time than that of a currently used CA125 IRMA kit which employed OC125 antibody both as 125I-labeled ligand and immunoadsorbent attached to beads. Almost identical CA125 values were obtained by using two kits. The antigenic nature recognized by the M-11 antibody seems substantially different from those of 130-22 or 145-9 antibodies recognizing CA130 antigen.
“SD - 8729”是一种一步法免疫放射分析试剂盒，它采用OC125抗体作为125I标记示踪剂，M - 11抗体作为免疫吸附剂。与目前使用的CA125免疫放射分析试剂盒相比，该试剂盒在较短孵育时间下就能观察到与磁珠结合的放射性更高，目前使用的试剂盒采用OC125抗体同时作为125I标记配体和附着在磁珠上的免疫吸附剂。使用这两种试剂盒获得的CA125值几乎相同。M - 11抗体识别的抗原性质似乎与识别CA130抗原的130 - 22或145 - 9抗体的抗原性质有很大不同。
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