Kakizaki J, Miyazaki T, Maeda T
Department of Radiology, Kyoto Prefectural University of Medicine.
Kaku Igaku. 1993 Jul;30(7):807-12.
We made basic studies of BALL ELSA CA125-II KIT which is a solid phase two site immunoradiometric assay. The first monoclonal antibody (M11) is coated on the BALL ELSA solid phase, and the second one (OC125), radiolabeled with 125I, is used as a tracer. Since serum CA125 values varied by incubation time, incubation temperature and agitation speed, the conditions of the assay had to be kept strictly. Intra- and interassay reproducibility, recovery test and dilution test were well satisfied. Prozone phenomenon was observed over 5,000 U/ml. The minimum detectable concentration was 7.5 U/ml. Serum CA125 values measured using BALL ELSA CA125-II KIT was correlated with those measured using conventional CENTOCOR CA125 RIA KIT (y = 1.058x-0.106 r = 0.973), although in the serum of a patient with chronic pancreatitis the discrepancy between serum CA125 values measured using these kits was observed.
我们对BALL ELSA CA125-II试剂盒进行了基础研究，该试剂盒是一种固相双位点免疫放射分析方法。第一种单克隆抗体（M11）包被在BALL ELSA固相上，第二种单克隆抗体（OC125）用125I进行放射性标记，用作示踪剂。由于血清CA125值会因孵育时间、孵育温度和振荡速度而变化，因此必须严格保持检测条件。批内和批间重复性、回收率试验和稀释试验均令人满意。在超过5000 U/ml时观察到前带现象。最低检测浓度为7.5 U/ml。使用BALL ELSA CA125-II试剂盒测定的血清CA125值与使用传统的CENTOCOR CA125 RIA试剂盒测定的值相关（y = 1.058x - 0.106，r = 0.973），尽管在一名慢性胰腺炎患者的血清中，观察到使用这些试剂盒测定的血清CA125值之间存在差异。
