Abacioğlu H, Yuluğ N
Dokuz Eylül Universitesi, Tip Fakültesi, Mikrobiyoloji Anabilim Dali.
Mikrobiyol Bul. 1993 Oct;27(4):284-93.
Escherichia coli hemolysin is one of the most important virulence determinants for uropathogenic strains. Hemolysin is cytotoxic to renal proximal tubular epithelial cells and leukocytes. It also causes the release of various mediators of inflammation and enzymes from leukocytes, thereby enhances the tissue damage to renal epithelium. The secretion kinetics of hemolysin was studied and found to be maximally secreted by an active process during logarithmic phase. The cytotoxic effect of the hemolysin was shown using Vero cells and by measuring Lactate Dehydrogenase activity in culture supernatants. The hemolytic strain showed significant cell lysis in comparison to non-hemolytic strain.
大肠杆菌溶血素是尿路致病性菌株最重要的毒力决定因素之一。溶血素对肾近端小管上皮细胞和白细胞具有细胞毒性。它还会导致白细胞释放各种炎症介质和酶，从而加剧对肾上皮的组织损伤。对溶血素的分泌动力学进行了研究，发现其在对数生长期通过一个活跃过程最大量地分泌。利用Vero细胞并通过测量培养上清液中的乳酸脱氢酶活性来显示溶血素的细胞毒性作用。与非溶血菌株相比，溶血菌株显示出明显的细胞裂解。
