Manna A C, Das H K
Genetic Engineering Unit, Jawaharlal Nehru University, New Delhi, India.
Mol Gen Genet. 1993 Dec;241(5-6):719-22. doi: 10.1007/BF00279916.
The chromosome of Azotobacter vinelandii UW was digested separately with the rape cutter restriction endonucleases SwaI (5'-ATTTAAAT), PmeI (5'GTTTAAAC) and PacI (5'-TTAATTAA) and the products were separated by pulsed-field gel electrophoresis. The size of the chromosome was determined to be approximately 4.5 megabase pairs (Mb) based on the sum of the sizes of the restriction fragments. This is almost the same as the size of the chromosome of Escherichia coli. The inability of the undigested DNA to enter the gel has led us to infer that the chromosome is circular.
用限制性内切酶SwaI（5'-ATTTAAAT）、PmeI（5'-GTTTAAAC）和PacI（5'-TTAATTAA）分别消化棕色固氮菌UW的染色体，产物通过脉冲场凝胶电泳进行分离。根据限制性片段大小总和，确定该染色体大小约为4.5兆碱基对（Mb）。这与大肠杆菌染色体大小几乎相同。未消化的DNA无法进入凝胶，这使我们推断该染色体是环状的。
