Parimala R, Sachdanandam P
Department of Medical Biochemistry, University of Madras, India.
Mol Cell Biochem. 1993 Aug 11;125(1):59-63. doi: 10.1007/BF00926835.
Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) isolated from Plumbago zeylanica Linn, when administered orally, at a dosage of 4 mg/kg body weight induces tumour regression in 3-methyl-4-dimethyl aminoazobenzene (3MeDAB) induced hepatoma in Wistar male rats. The purpose of this investigation was to identify the changes in the rate of glycolysis and gluconeogenesis in tumour-bearing rats and the effects of treatment with Plumbagin. The levels of certain glycolytic enzymes, namely, hexokinase; phosphoglucoisomerase; and aldolase levels increased (p < 0.001) in hepatoma bearing rats, whereas they decreased in Plumbagin administered rats to near normal levels. Certain gluconeogenic enzymes, namely, glucose-6-phosphatase and fructose-1,6-diphosphatase decreased (p < 0.001) in tumour hosts, whereas Plumbagin administration increased the gluconeogenic enzyme levels in the treated animals. These investigations indicate the molecular basis of the different biological behaviour of 3MeDAB induced hepatoma and the anticarcinogenic property of Plumbagin against hepatoma studied in rats.
从白花丹中分离出的白花丹素（5-羟基-2-甲基-1,4-萘醌），当以4毫克/千克体重的剂量口服给药时，可使Wistar雄性大鼠中由3-甲基-4-二甲基氨基偶氮苯（3MeDAB）诱导的肝癌发生肿瘤消退。本研究的目的是确定荷瘤大鼠糖酵解和糖异生速率的变化以及白花丹素治疗的效果。某些糖酵解酶的水平，即己糖激酶、磷酸葡萄糖异构酶和醛缩酶水平在荷肝癌大鼠中升高（p < 0.001），而在给予白花丹素的大鼠中它们降至接近正常水平。某些糖异生酶，即葡萄糖-6-磷酸酶和果糖-1,6-二磷酸酶在肿瘤宿主中降低（p < 0.001），而给予白花丹素可使治疗动物中的糖异生酶水平升高。这些研究表明了3MeDAB诱导的肝癌不同生物学行为的分子基础以及白花丹素对大鼠肝癌的抗癌特性。
