Bruatto M, Gremmi M, Nardacchione A, Amerio M
Laboratorio di Micologia Medica, Istituto Malattie Infettive dell'Università di Torino, Italy.
Mycopathologia. 1993 Aug;123(2):105-10. doi: 10.1007/BF01365088.
By incubating starved and unstarved yeast cells in synthetic media with a pH of 4.5 or 6.7 at 37 degrees C the effect of a 3 hours' glucose starvation on germ-tube production by Candida albicans was evaluated. In addition the endocellular content of total carbohydrates, glycogen, trehalose and proteins after and before the starvation were dosed. The most interesting result was the overcoming of the pH-regulated dimorphism, thanks to the starvation treatment. In fact the starved cultures produced germ-tubes indifferently in neutral or acid media, whereas the filamentation of the unstarved cultures was more copious in pH 6.7 medium. The endocellular content of trehalose and protein was unchanged, whereas total carbohydrates and glycogen showed a shortage after the 3 hours' glucose starvation. The possible involvements of these metabolic changes in the regulation of dimorphic transition are discussed.
通过在37摄氏度下，将饥饿和未饥饿的酵母细胞在pH值为4.5或6.7的合成培养基中培养，评估3小时葡萄糖饥饿对白色念珠菌芽管产生的影响。此外，还测定了饥饿前后细胞内总碳水化合物、糖原、海藻糖和蛋白质的含量。最有趣的结果是，饥饿处理克服了pH调节的二态性。事实上，饥饿培养物在中性或酸性培养基中均可产生芽管，而未饥饿培养物在pH 6.7的培养基中丝状化更为丰富。海藻糖和蛋白质的细胞内含量未发生变化，而3小时葡萄糖饥饿后总碳水化合物和糖原含量减少。讨论了这些代谢变化在双态转变调节中的可能作用。
