Cho T, Hamatake H, Kaminishi H, Kuroki A, Suehara T, Suehara Y, Sakima T, Hagihara Y, Watanabe K
Fukuoka Shika Daigaku Gakkai Zasshi. 1989;16(4):510-21.
Two chemically defined media were developed for the induction of germ tubes in exponential phase cells of Candida albicans. One medium was N-acetyl-D-glucosamine medium which is composed of L-thiazolidine-4-carboxylic acid, L-proline, NaHCO3, sodium acetate, NaH2PO4 and N-acetyl-D-glucosamine. The other one was glucose medium in which N-acetyl-D-glucosamine is exchanged for glucose plus NH4Cl in N-acetyl-D-glucosamine medium. In these media, a high percentage of germ tube forming cells was obtained without a temperature shift. However, starvation of the cells in water at 37 degrees C was a necessary pretreatment to consistently obtain a high percentage of germ tube forming cells. The effect of starvation was remarkable in glucose medium, the percentages of germ tube forming cells among the normal cells and starved cells were 20 and 80, respectively. As for intracellular changes during starvation, a decrease in adenosine triphosphate concentration and an increase in adenosine 3',5'-cyclic monophosphate concentration were observed.
开发了两种化学成分明确的培养基，用于诱导白色念珠菌指数生长期细胞形成芽管。一种培养基是N-乙酰-D-葡萄糖胺培养基，它由L-噻唑烷-4-羧酸、L-脯氨酸、NaHCO₃、乙酸钠、NaH₂PO₄和N-乙酰-D-葡萄糖胺组成。另一种是葡萄糖培养基，其中在N-乙酰-D-葡萄糖胺培养基中用葡萄糖加NH₄Cl替代了N-乙酰-D-葡萄糖胺。在这些培养基中，无需温度变化就能获得高比例的芽管形成细胞。然而，将细胞在37℃的水中饥饿处理是持续获得高比例芽管形成细胞的必要预处理。饥饿处理在葡萄糖培养基中的效果显著，正常细胞和饥饿细胞中芽管形成细胞的比例分别为20%和80%。至于饥饿期间的细胞内变化，观察到三磷酸腺苷浓度降低，3',5'-环磷酸腺苷浓度升高。
