Shepherd M G, Yin C Y, Ram S P, Sullivan P A
Can J Microbiol. 1980 Jan;26(1):21-6. doi: 10.1139/m80-004.
A reproducible and simple system for the production of germ tubes from yeast cells of Candida albicans using glucose and glutamine as substrates has been described. During germ tube formation there was a doubling of the dry weight but the number of cells remained constant. Although the DNA content did not change for the first 4 h of germ tube formation, the RNA content more than doubled. The DNA and RNA content of C. albicans blastospores are 4.5 x 10(-15) g per cell and 48 x 10(-15) g per cell respectively. Nystatin, phenethyl alcohol, 2,4-dinitrophenol, azaserine, salicylhydroxamic acid, and 5-fluorocytosine were all effective inhibitors of germ tube formation. Cysteine, potassium cyanide, and polyoxin D did not prevent germination. The incorporation of both uracil and leucine occurred rapidly during germ tube formation. The inhibitors of RNA synthesis, actinomycin D. cordycepin, and daunomycin prevented germination and inhibited uracil incorporation. The translational inhibitors, trichodermin, aurin tricarboxylic acid, puromycin, and cyloheximide were effective in inhibiting both germ tube formation and leucine incorporation.
已经描述了一种可重复且简单的系统，该系统使用葡萄糖和谷氨酰胺作为底物，从白色念珠菌的酵母细胞中产生芽管。在芽管形成过程中，干重增加了一倍，但细胞数量保持不变。尽管在芽管形成的最初4小时内DNA含量没有变化，但RNA含量增加了一倍多。白色念珠菌芽生孢子的DNA和RNA含量分别为每个细胞4.5×10(-15)克和48×10(-15)克。制霉菌素、苯乙醇、2,4-二硝基苯酚、重氮丝氨酸、水杨羟肟酸和5-氟胞嘧啶都是芽管形成的有效抑制剂。半胱氨酸、氰化钾和多氧霉素D不能阻止发芽。在芽管形成过程中，尿嘧啶和亮氨酸的掺入都迅速发生。RNA合成抑制剂放线菌素D、虫草素和柔红霉素阻止发芽并抑制尿嘧啶掺入。翻译抑制剂木霉菌素、金精三羧酸、嘌呤霉素和环己酰亚胺可有效抑制芽管形成和亮氨酸掺入。
