O'Conner J L, Wade M F, Brann D W, Mahesh V B
Department of Physiology and Endocrinology, Medical College of Georgia, Australia 30912.
Neuroendocrinology. 1993 Jul;58(1):129-35. doi: 10.1159/000126521.
Neuropeptide Y (NPY) acts at the hypothalamus to stimulate LH secretion through increased LHRH secretion. NPY is also cosecreted into the portal system in a pulsatile manner with LHRH and may act at the anterior pituitary to increase LH secretion through potentiation of LHRH responsiveness and possibly through direct stimulation; however, controversy exists concerning this direct action of NPY at the pituitary. Utilizing dispersed anterior pituitary monolayer cell cultures, the first goal of this study was to determine the effects of vehicle, 10(-10) M LHRH, 10(-7) M NPY, 10(-6) M NPY, 10(-10) M LHRH + 10(-7) M NPY, and 10(-10) M LHRH + 10(-6) M NPY on LH and FSH responsiveness. Under these conditions, 10(-6) M NPY alone significantly stimulated LH secretion (630% above basal) and both 10(-7) M NPY and 10(-6) M NPY significantly potentiated LHRH-induced LH secretion (195 and 244% above LHRH alone, respectively). Neither 10(-7) M NPY nor 10(-6) M NPY alone stimulated FSH secretion; however, both 10(-7) M and 10(-6) M NPY significantly potentiated LHRH-induced FSH secretion (130 and 135% above LHRH alone, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
神经肽Y（NPY）作用于下丘脑，通过增加促性腺激素释放激素（LHRH）的分泌来刺激促黄体生成素（LH）的分泌。NPY还与LHRH以脉冲方式共同分泌到门脉系统中，并可能作用于垂体前叶，通过增强LHRH反应性以及可能通过直接刺激来增加LH分泌；然而，关于NPY在垂体的这种直接作用存在争议。利用分散的垂体前叶单层细胞培养，本研究的首要目标是确定溶剂、10⁻¹⁰ M LHRH、10⁻⁷ M NPY、10⁻⁶ M NPY、10⁻¹⁰ M LHRH + 10⁻⁷ M NPY以及10⁻¹⁰ M LHRH + 10⁻⁶ M NPY对LH和促卵泡生成素（FSH）反应性的影响。在这些条件下，单独使用10⁻⁶ M NPY可显著刺激LH分泌（比基础水平高630%），10⁻⁷ M NPY和10⁻⁶ M NPY均显著增强LHRH诱导的LH分泌（分别比单独使用LHRH高195%和244%）。单独使用10⁻⁷ M NPY和10⁻⁶ M NPY均未刺激FSH分泌；然而，10⁻⁷ M和10⁻⁶ M NPY均显著增强LHRH诱导的FSH分泌（分别比单独使用LHRH高130%和135%）。（摘要截选至250字）
