Woller M J, Campbell G T, Liu L, Steigerwalt R W, Blake C A
Department of Cell Biology and Neuroscience, University of South Carolina School of Medicine, Columbia 29208.
Endocrinology. 1993 Dec;133(6):2675-81. doi: 10.1210/endo.133.6.8243291.
In recent years, several studies have shown that neuropeptide-Y (NPY) is involved in the control of LH secretion. We determined the effects of estrogen on NPY-induced LH and FSH release in the absence or presence of LH-releasing hormone (LHRH) at the level of the anterior pituitary gland (APG). Adult female rats were ovariectomized. Fifteen to 20 days later, they were given a blank or estrogen-filled capsule subdermally and killed 17-19 h later. APG cells were isolated and cultured for 3 days in medium containing 12.5% rat serum collected at death from the same rats used to make the respective APG cell pools. The cells were then challenged for 3 h with vehicle, NPY (10(-12)-10(-6) M), LHRH (10(-9)-10(-6) M), or combinations of NPY (10(-9)-10(-7) M) and LHRH (10(-9) M). LHRH stimulated LH and FSH release from nonestrogen and estrogen-primed cells. NPY at 6.7 x 10(-8)-10(-6) M increased (P < 0.05) LH release and at 10(-6) M increased (P < 0.05) FSH release from estrogen-primed cells, but was without effect on nonestrogen-primed cells. In contrast, NPY at 10(-9)-10(-7) M potentiated the action of LHRH (10(-9) M) to increase the release of LH and FSH from nonestrogen-primed cells, but was without potentiating effects in cultures of estrogen-primed cells. The results demonstrate that 1) NPY can release LH and FSH by a direct action on estrogen-primed APG cells; and 2) NPY can potentiate the action of LHRH to increase the release of LH and FSH by a direct action on nonestrogen-primed APG cells.
近年来，多项研究表明神经肽Y（NPY）参与促黄体生成素（LH）分泌的调控。我们在垂体前叶水平测定了雌激素在有无促性腺激素释放激素（LHRH）存在的情况下对NPY诱导的LH和促卵泡激素（FSH）释放的影响。成年雌性大鼠接受卵巢切除术。15至20天后，给它们皮下植入空白或含雌激素的胶囊，并在17 - 19小时后处死。分离垂体前叶细胞，在含有从用于制备相应垂体前叶细胞池的同一只大鼠死亡时采集的12.5%大鼠血清的培养基中培养3天。然后用溶剂、NPY（10⁻¹² - 10⁻⁶ M）、LHRH（10⁻⁹ - 10⁻⁶ M）或NPY（10⁻⁹ - 10⁻⁷ M）与LHRH（10⁻⁹ M）的组合对细胞进行3小时刺激。LHRH刺激未用雌激素处理和经雌激素预处理的细胞释放LH和FSH。6.7×10⁻⁸ - 10⁻⁶ M的NPY可增加（P < 0.05）经雌激素预处理细胞的LH释放，10⁻⁶ M的NPY可增加（P < 0.05）经雌激素预处理细胞的FSH释放，但对未用雌激素处理的细胞无影响。相反，10⁻⁹ - 10⁻⁷ M的NPY增强了LHRH（10⁻⁹ M）增加未用雌激素处理细胞释放LH和FSH的作用，但对经雌激素预处理细胞的培养物无增强作用。结果表明：1）NPY可通过直接作用于经雌激素预处理的垂体前叶细胞释放LH和FSH；2）NPY可通过直接作用于未用雌激素处理的垂体前叶细胞增强LHRH增加LH和FSH释放的作用。
