c-Myc/Max和Max/Max二聚体不同的DNA结合偏好。
Distinct DNA binding preferences for the c-Myc/Max and Max/Max dimers.
作者信息
Solomon D L, Amati B, Land H
机构信息
Growth Control and Development Laboratory, Imperial Cancer Research Fund, London, UK.
出版信息
Nucleic Acids Res. 1993 Nov 25;21(23):5372-6. doi: 10.1093/nar/21.23.5372.
Abstract
The transcription factor c-Myc and its dimerisation partner Max are members of the basic/helix-loop-helix/leucine-zipper (bHLH-Z) family and bind to the DNA core sequence CACGTG. Using a site-selection protocol, we determined the complete 12 base pair consensus binding sites of c-Myc/Max (RACCACGTGGTY) and Max/Max (RANCACGTGNTY) dimers. We find that the c-Myc/Max dimer fails to bind the core when it is flanked by a 5'T or a 3'A, while the Max/Max dimer readily binds such sequences. Furthermore we show that inappropriate flanking sequences preclude transactivation by c-Myc in vivo. In conclusion, Max/Max dimers are less discriminatory than c-Myc/Max and may regulate other genes in addition to c-Myc/Max targets.
摘要
转录因子c-Myc及其二聚化伴侣Max是碱性/螺旋-环-螺旋/亮氨酸拉链（bHLH-Z）家族的成员，可与DNA核心序列CACGTG结合。我们使用位点选择方案确定了c-Myc/Max（RACCACGTGGTY）和Max/Max（RANCACGTGNTY）二聚体完整的12个碱基对共有结合位点。我们发现，当c-Myc/Max二聚体的侧翼为5'T或3'A时，它无法结合核心序列，而Max/Max二聚体则能轻易结合此类序列。此外，我们还表明，不合适的侧翼序列会在体内阻止c-Myc的反式激活。总之，Max/Max二聚体的特异性不如c-Myc/Max，除了c-Myc/Max的靶标外，还可能调控其他基因。
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本文引用的文献
1
2
c-Myc trans-activates the p53 promoter through a required downstream CACGTG motif.c-Myc通过一个必需的下游CACGTG基序反式激活p53启动子。
Cell Growth Differ. 1993 Feb;4(2):57-65.
3
Recognition by Max of its cognate DNA through a dimeric b/HLH/Z domain.Max通过二聚体b/HLH/Z结构域识别其同源DNA。
Nature. 1993 May 6;363(6424):38-45. doi: 10.1038/363038a0.
4
Alternative mRNA forms and open reading frames of the max gene.max基因的可变mRNA形式和开放阅读框。
Oncogene. 1993 Feb;8(2):503-7.
5
Oncogenic activity of the c-Myc protein requires dimerization with Max.c-Myc蛋白的致癌活性需要与Max形成二聚体。
Cell. 1993 Jan 29;72(2):233-45. doi: 10.1016/0092-8674(93)90663-b.
6
Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites.Mxi1是一种与Max特异性相互作用以结合Myc-Max识别位点的蛋白质。
Cell. 1993 Jan 29;72(2):223-32. doi: 10.1016/0092-8674(93)90662-a.
7
Mad: a heterodimeric partner for Max that antagonizes Myc transcriptional activity.Mad：一种与Max形成异二聚体的伙伴，可拮抗Myc转录活性。
Cell. 1993 Jan 29;72(2):211-22. doi: 10.1016/0092-8674(93)90661-9.
8
9
Sequence-specific transcriptional activation by Myc and repression by Max.Myc介导的序列特异性转录激活以及Max介导的转录抑制。
Mol Cell Biol. 1993 Jan;13(1):383-90. doi: 10.1128/mcb.13.1.383-390.1993.
10
Opposite regulation of gene transcription and cell proliferation by c-Myc and Max.c-Myc和Max对基因转录和细胞增殖的相反调节作用。
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