Perez-Albuerne E D, Schatteman G, Sanders L K, Nathans D
Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205-2185.
Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11960-4. doi: 10.1073/pnas.90.24.11960.
JunB is an immediate early transcription factor that is induced by a variety of extracellular signaling agents, including growth factors, phorbol esters, and agents that elevate cyclic AMP. The mechanism of activation of the gene encoding JunB by these agents is not well understood. By using the JunB gene together with flanking DNA in transfection experiments, we show that a serum response element (SRE) and/or a cAMP response element (CRE) downstream of the gene mediate the response of the gene in mouse NIH 3T3 cells to serum, platelet-derived growth factor, basic fibroblast growth factor, phorbol ester, and forskolin. In addition, a segment of DNA just upstream of the TATA box is required for optimal activation of the gene.
JunB是一种即时早期转录因子，可由多种细胞外信号传导因子诱导产生，包括生长因子、佛波酯以及能提高环磷酸腺苷水平的因子。目前对这些因子激活JunB编码基因的机制了解尚少。在转染实验中，我们将JunB基因及其侧翼DNA一同使用，结果表明该基因下游的血清反应元件（SRE）和/或环磷酸腺苷反应元件（CRE）介导了小鼠NIH 3T3细胞中该基因对血清、血小板衍生生长因子、碱性成纤维细胞生长因子、佛波酯和福斯高林的反应。此外，TATA框上游的一段DNA序列对于该基因的最佳激活是必需的。
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