Sirum-Connolly K, Mason T L
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003.
Science. 1993 Dec 17;262(5141):1886-9. doi: 10.1126/science.8266080.
The product of the PET56 nuclear gene of Saccharomyces cerevisiae was shown to be required for ribose methylation at a universally conserved nucleotide in the peptidyl transferase center of the mitochondrial large ribosomal RNA (21S rRNA). Cells reduced in this activity were deficient in formation of functional large subunits of the mitochondrial ribosome. The purified Pet56 protein catalyzed the site-specific formation of 2'-O-methylguanosine on in vitro transcripts of both mitochondrial 21S rRNA and Escherichia coli 23S rRNA. These results provide evidence for an essential modified nucleotide in rRNA.
酿酒酵母PET56核基因的产物被证明是线粒体大核糖体RNA（21S rRNA）肽基转移酶中心一个普遍保守核苷酸处核糖甲基化所必需的。这种活性降低的细胞在线粒体核糖体功能大亚基的形成上存在缺陷。纯化的Pet56蛋白催化线粒体21S rRNA和大肠杆菌23S rRNA体外转录本上2'-O-甲基鸟苷的位点特异性形成。这些结果为rRNA中一种必需的修饰核苷酸提供了证据。
